產品更新-人胃粘膜上皮細胞

培養基 DMEM-H完(wan)-全培養基：90%DMEM-H+10%FBS

傳代方(fang)法 復蘇步驟：①凍存管(guan)從液(ye)氮(dan)或-80℃冰箱中(zhong)(zhong)取出放(fang)入(ru)(ru)(ru)PE手(shou)套中(zhong)(zhong)，迅速(su)沒入(ru)(ru)(ru)37℃水浴鍋，搖(yao)晃凍存管(guan)加(jia)速(su)溶(rong)解，以1min內(nei)全(quan)(quan)部溶(rong)解為(wei)宜；②在(zai)超凈臺中(zhong)(zhong)將溶(rong)解好的細(xi)胞液(ye)加(jia)入(ru)(ru)(ru)到裝有9mL完(wan)-全(quan)(quan)培養(yang)(yang)基(ji)的離(li)(li)心管(guan)內(nei)，1000-1200rpm離(li)(li)心5min，棄去上清液(ye)，用1-2mL完(wan)-全(quan)(quan)培養(yang)(yang)基(ji)重懸(xuan)細(xi)胞。③將細(xi)胞懸(xuan)液(ye)加(jia)入(ru)(ru)(ru)到含有5-6mL完(wan)-全(quan)(quan)培養(yang)(yang)基(ji)的T25瓶中(zhong)(zhong)，放(fang)入(ru)(ru)(ru)培養(yang)(yang)箱培養(yang)(yang)。

細(xi)(xi)(xi)(xi)(xi)胞(bao)傳代：①將舊培養液(ye)(ye)吸除，PBS清洗(xi)兩遍(bian)后，加入(ru)1-2mL胰(yi)酶(mei)（0.25%Trypsin+0.02%EDTA）；②鏡(jing)下(xia)觀察消(xiao)化(hua)(hua)情(qing)況(kuang)，在細(xi)(xi)(xi)(xi)(xi)胞(bao)邊緣縮小(xiao)，貼(tie)壁(bi)松動時(shi)（可用吸管吸起些(xie)許(xu)胰(yi)酶(mei)輕輕吹打細(xi)(xi)(xi)(xi)(xi)胞(bao)層(ceng)(ceng)某處，肉(rou)眼可見(jian)細(xi)(xi)(xi)(xi)(xi)胞(bao)層(ceng)(ceng)脫落，即消(xiao)化(hua)(hua)完(wan)(wan)(wan)成(cheng)，否則繼續消(xiao)化(hua)(hua)），直(zhi)接吸掉(diao)胰(yi)酶(mei)，加入(ru)5-6mL完(wan)(wan)(wan)-全(quan)培養基(ji)，輕輕吹打細(xi)(xi)(xi)(xi)(xi)胞(bao)層(ceng)(ceng)，把(ba)細(xi)(xi)(xi)(xi)(xi)胞(bao)層(ceng)(ceng)吹落，吹散(san)。③將細(xi)(xi)(xi)(xi)(xi)胞(bao)懸液(ye)(ye)按1:2比(bi)例分到新的T25瓶(ping)中(zhong)，添加適當(dang)的完(wan)(wan)(wan)-全(quan)培養基(ji)，把(ba)細(xi)(xi)(xi)(xi)(xi)胞(bao)懸液(ye)(ye)打勻，于培養箱中(zhong)培養。；④注意(yi)培養基(ji)pH值(zhi)變化(hua)(hua)和細(xi)(xi)(xi)(xi)(xi)胞(bao)密度(du)，定期換液(ye)(ye)（每周2-3次），待細(xi)(xi)(xi)(xi)(xi)胞(bao)密度(du)達到80%-90%時(shi)，重復傳代操(cao)作或者(zhe)凍(dong)存(cun)。

生長條件 37℃；5%CO2+95%空氣；
生長特性 貼壁生長
存儲條件 液氮
安全等級 1
形(xing)態 上皮細胞樣，多角(jiao)形(xing)，邊緣整齊，單層貼壁生長，背景干凈，不產(chan)色(se)素，無空(kong)泡